-
Melt low-melt fragment at 65°C, ~5’.
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Approximate volume and add 1/10 volume of 10x Beta-agarose
buffer. Mix well.
-
Cool to 40°C in a temp. block. Add 2 U Beta-agarose
per 200 µl agarose. Mix.
-
Incubate at 40°C for 1-2 hrs.
-
Add 1/10 volume 3M NaOAc. Mix. Incubate on ice 15’.
Move to a fresh tube, avoiding undigested agarose, after a 10’ spin at
max.
-
Precipitate with 2 volumes isopropanol. Mix and chill
at -20°C for 30’.
-
Centrifuge 15’ at max. Remove supernatant and wash with
1 ml 70% EtOH.
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