Disodium Ethylenedeamine tetraacetate • 2H₂O   186.12 g

• C₁₀H₁₄O₈Na₂N₂ • 2H₂O-----372.24 g (MW)
• use EDTA Disodium Salt from Fisherbiotech

• Total Volume:                                            1000.0 ml

• After 700 ml of dH₂O is added--stir, adjust pH to 8.0 then fill up to 1000.0 ml. Note: EDTA will not dissolve until pH approaches 8.

• Aliquot 100 ml into (10) 200 ml square bottles or...

• Aliquot 500 ml into (2) 1000 ml bottles.