Current Lab Chemical List
Protocols
Electron Microscopy
Scanning Electron Microscopy
Transmission Electron Microscopy
Molecular Techniques (DNA/RNA)
Alkaline Lysis Mini-Prep
BAC (small) DNA prep
BAC (large) DNA prep
Beta-agarose digestion
Blue white selection
Calcium Comp Transformation
Colony PCR
Comp. Cell Prep for Elec.
Exol-SAP Prep for Sequencing
Hexamer Labeling
High Throughput Sequencing
Northern
PCR Protocol
Plant DNA Prep Using Large Scale CTAB
Prep & Use Frozen E coli
Restriction Enzyme Digest
RNA extraction
RT - PCR
Shorty DNA Prep
Shotgun Library
Shrimp Alkaline Phosphatase
Southern
TAIL PCR protocol
Topo cloning
Transform of Electrocomp Cells
Protein Protocols
Dry Transfer
Protein Concentration Assay
Western Protocol
Plant Protocols
Analine Blue Staining
Basta Selection
Complementation in Arabidopsis
DAPI
FDA
GUS Staining of Pollen
Planting Seed
Pollen Growth Media
Soil Prep
Sterilizing Seed
Stock sol & Lab procedures
0.1% Agar
0.5 M EDTA (pH 8)
1.0 M Calcium Chloride
1.0 M Magnesium Sulfate
10% SDS
2.0 M Tris Buffer
20% SDS
3.0 M NaOAc
4.0 M NaOH
4.0M KOH
40% Glucose
5.0 M NaCl
Acrylamide Prep
Antibiotic Concentrations
ddWater
LB Media
MS Media
MS Media Alternative
PBS (pH 7.4)
SD Media
SOB Media
TAE-(stock & gel buffer)
Top Agar
YEP Media