SD Media
Synthetic Minimal Media: Stock Solution


Plates Liquid
Yeast Nitrogen Base 6.7 g 6.7 g
Bacto Agar 20.0 g NONE
Deionized H2O 800.0 ml 800.0 ml
* Total Volume: 1000.0 ml 1000.0 ml
     
    • After 800 ml of dH2O is added--stir, then fill up to 1000.0 ml.
     
    • Aliquot 100 ml into (10) 200 ml square bottles.(For liquuid only)
     
    • Sterilize by autoclaving 30 minutes (liquid cycle).